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En protokol til at inficere<em> Caenorhabditis elegans</em> Med<em> Salmonella typhimurium</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium
DOI:

06:50 min

June 26, 2014

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Chapters

  • 00:05Title
  • 00:53Preparations
  • 02:01Feeding RNAi to Nematodes
  • 03:34Treating C. elegans with Salmonella and Scoring Survival
  • 05:41C. elegans Survival from Salmonella Infection and bec-1
  • 06:25Conclusion

Summary

Automatic Translation

C. elegans har vist sig som en ny genetisk model til at studere vært-patogen interaktioner. Her beskriver vi en protokol til at inficere C. elegans med Salmonella typhimurium kombineret med den dobbelt-strenget RNAi interferens teknik til at undersøge, hvilken rolle værtsgener i forsvaret mod Salmonella infektion.

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