A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Plastic Surgery and Hand Surgery, Klinikum rechts der Isar,Technische Universität München, 2Department of Plastic, Reconstructive, Aesthetic and Hand Surgery,University Hospital of Basel, 3Institute for Clinical and Experimental Surgery,University of Saarland, 4Division of Plastic and Hand Surgery,University Hospital Zurich
Chapters
- 00:05Title
- 01:35Animal Preparation and Surgical Elevation of the Flap
- 06:09Intravital Epifluorescence Microscopy
- 07:57Results: Tissue Remodeling in Response to Acute Persistent Ischemia
- 09:42Conclusion
Vinduet av det murine dorsal skinfold kammeret presentert visualiserer en sone av akutt iskemi vedvarende over en musculocutaneous klaff. Intra epi-fluorescens mikrostillatelse for direkte og repeterende vurdering av microvasculature og kvantifisering av hemodynamics. Morfologisk og hemodynamiske resultater kan videre være korrelert med molekyl og histologiske analyser.
