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Real-time cytotoksicitetsassays i humant fuldblod
JoVE Journal
Medicine
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JoVE Journal Medicine
Real-time Cytotoxicity Assays in Human Whole Blood
DOI:

08:27 min

November 07, 2014

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Chapters

  • 00:05Title
  • 01:05Experimental Setup and Target Cell Preparation
  • 02:46Preparation of Cell Microarrays
  • 04:05Whole Blood Cytotoxicity Assays
  • 06:03Measuring Raji Cell Death via WCA
  • 07:55Conclusion

Summary

Automatic Translation

Helblodet cytotoksicitetsassay (WCA) er en cytotoksicitetsanalyse udviklet ved at inkorporere high-throughput celle positionering teknologi med fluorescensmikroskopi og automatiseret billedbehandling. Her beskriver vi, hvordan lymfom celler behandlet med et anti-CD20-antistof kan analyseres i realtid i humant fuldblod give kvantitative cellulær cytotoksicitet analyse.

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