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Journal / Neuroscience / Vibratome Sektionsinddeling Mouse Retina Forbered …
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Neuroscience

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JoVE Journal Neuroscience
Vibratome Sektionsinddeling Mouse Retina Forbered fotoreceptoren kulturer
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Journal (Neuroscience)
Spinal Cord Neurons Isolation and Culture from Neonatal Mice
Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester
Journal (Neuroscience)
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Vibratome Sektionsinddeling Mouse Retina Forbered fotoreceptoren kulturer

Article DOI: 10.3791/51954-v • 11:22 min • December 22nd, 2014
December 22nd, 2014 •
1,4,5,6, 1,4,5,6, 2,4,5,6, 3,4,5,6, 4,5,6, 1,4,5,6
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

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Neurale nethinde af en mus i alderen 8 dage er på toppen af ​​en 4% gelatine blok. Efter isolering af fotoreceptorlaget (200 um) ved vibratome er fotoreceptorerne podes efter mekanisk og enzymatisk dissociation for kulturen. Fotoreceptorlaget kan anvendes til molekylære biokemiske analyser eller transplantation.

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Medicine Primær fotoreceptor cellekultur arvet retinal degeneration Rod-afledte Cone Viability Factor S-antigen Fladskærms-monterede mus nethinden Transplantation.
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