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JoVE Journal Neuroscience
To peelingmetoder til isolering af fotoreceptorcellerum i musenehinden til proteinanalyse
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To peelingmetoder til isolering af fotoreceptorcellerum i musenehinden til proteinanalyse

Article DOI: 10.3791/62977-v • 11:08 min • December 7th, 2021
December 7th, 2021 •
1, 1, 1,2
1Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, 2Department of Cell & Neurobiology, Keck School of Medicine, University of Southern California

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Denne protokol præsenterer to teknikker til at isolere de subcellulære rum af murine stang fotoreceptorer til proteinanalyse. Den første metode bruger levende nethinde- og cellulosefilterpapir til at adskille stangens ydre segmenter, mens den anden anvender lyofiliseret nethinde og klæbebånd til at skrælle stangens indre og ydre segmentlag væk.

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Neurovidenskab udgave 178
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