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从开发小脑显微切割分离的不同的细胞群
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Please note that all translations are automatically generated.
Click here for the English version.
从开发小脑显微切割分离的不同的细胞群
DOI:
10.3791/52034-v
•
08:02 min
•
September 21, 2014
•
Larra W. Yuelling
,
Fang Du
,
Peng Li
,
Renata E. Muradimova
,
Zeng-jie Yang
1
Cancer Biology Program, Fox Chase Cancer Center
,
Temple University Health System
Chapters
00:05
Title
01:09
General Preparations
02:41
Dissection and Preparation of Tissue Slices
04:57
Tissue Microdissection and Cell Dissociation, Sorting, and Plating
06:46
Results of Isolating and Culturing NEPs
07:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
巢蛋白表达祖细胞是在发展中国家小脑的神经元祖细胞的新发现的种群。用在这里用荧光激活细胞分选组合呈现的显微切割技术中,该细胞群可与任何其他小脑区域污染被纯化和可以培养用于进一步研究。
Tags
Microdissection
Nestin-expressing Progenitors (NEPs)
Cerebellar External Germinal Layer (EGL)
Fluorescent-activated Cell Sorting (FACS)
Cell Isolation
Cell Culture
Percoll Gradient Centrifugation
Laser Capture Microdissection
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