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Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
JoVE Journal
Biology
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JoVE Journal Biology
Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

DOI:
10.3791/52223-v

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10:57 min

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December 16, 2014

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, ,
1Institut für Biochemie und Molekulare Biologie,Universität Ulm, 2Institut für Immunologie,Universitätsklinikum Ulm

Chapters

  • 00:05Title
  • 01:31Preparation of the Gastrointestinal Tract and Dissection of the Midgut
  • 03:14Digestion of the Midgut Tissue to Harvest Cells for FAC Sorting
  • 05:25FACS to Isolate Intestinal Stem Cells (ISC)
  • 08:52Results: Representative Analyses of the Sorted Cells
  • 10:17Conclusion

Summary

Automatic Translation

Automatic Translation

Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.

Tags

Keywords: Intestinal Stem CellsDrosophilaFACSAgingMidgutEnteroblastGFPCell Isolation

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