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Elektrofore Mobility Shift Assay (EMSA) för studier av RNA-proteininteraktioner
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Elektrofore Mobility Shift Assay (EMSA) för studier av RNA-proteininteraktioner: The IRE / IRP Exempel

Article DOI: 10.3791/52230-v • 12:44 min • December 3rd, 2014
December 3rd, 2014 •
1,2, 1,2, 1,2
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Department of Medicine, McGill University

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Här presenterar vi ett protokoll för att analysera RNA / proteininteraktioner. Den elektroforetiska rörligheten shift assay (EMSA) är baserad på den differentiella migreringen av RNA / proteinkomplex och fri RNA under nativ gelelektrofores. Genom att använda en radiomärkt RNA-prob, kan RNA / proteinkomplex visualiseras genom autoradiografi.

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Biokemi RNA metabolism mRNA translation post-transkription genreglering mRNA stabilitet IRE IRP1 IRP2 järnmetabolism ferritin transferrinreceptor
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