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Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes
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Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes

Techniques for Imaging Prometaphase and Metaphase of Meiosis I in Fixed Drosophila Oocytes

DOI:
10.3791/54666-v

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09:11 min

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October 31, 2016

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,
1Waksman Institute,Rutgers University, 2Department of Genetics,Rutgers University

Chapters

  • 00:05Title
  • 00:36Collection of Late-stage Drosophila Oocytes
  • 02:21Fixation of Oocyte Tissue and Membrane Removal (“Rolling”)
  • 04:09Antibody Staining of Drosophila Oocytes
  • 06:47Results: Visualization of Prophase and Metaphase in Drosophila Oocytes
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

We present protocols for the collection, preparation, and imaging of mature Drosophila oocytes. These methods allow the visualization of chromosome behavior and spindle assembly and function during meiosis.

Tags

Keywords: MeiosisDrosophila OocytesSpindle AssemblyChromosome OrientationImmunofluorescenceROB’s BufferOocyte IsolationHeptanePBS

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