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JoVE Journal Biology
Mätning av mRNA dämpfaktorer i
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Mätning av mRNA dämpfaktorer i

Article DOI: 10.3791/52240
December 13th, 2014 •
1, 1
1Department of Biology, Baylor University

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December 13th, 2014

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Steady state-nivån av specifika mRNA bestäms av graden av syntes och nedbrytning av mRNA. Genomvid mRNA nedbrytningshastigheter eller dämpfaktorer specifika mRNA kan mätas genom att bestämma mRNA halveringstider. Detta protokoll är inriktat på mätning av mRNA avklingningshastigheter i Saccharomyces cerevisiae.

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