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mRNA的衰变率的测量<em>酿酒酵母</em>使用<em> rpb1-1</em>株
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
DOI:

12:21 min

December 13, 2014

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Chapters

  • 00:05Title
  • 01:22Growth of Yeast Cells to Log Phase
  • 02:21Heat-induced Inhibition of mRNA Synthesis and Harvest of Yeast Cells
  • 05:18RNA Gel Electrophoresis and Northern Blot Transfer
  • 07:07Northern Hybridization
  • 08:56RNA Quantitation
  • 10:03Results: Down-regulation of an Intron Containing Pre-mRNA in NMD Mutants
  • 11:59Conclusion

Summary

Automatic Translation

特定的mRNA的稳态水平由合成的mRNA和衰减率决定。的全基因组的mRNA的降解率或特异mRNAs的衰变率可通过测定mRNA的半衰期进行测定。该协议的重点是mRNA降解率在酿酒酵母中的测量。

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