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Journal / Biology / Meting van mRNA Decay tarieven in
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Meting van mRNA Decay tarieven in
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Meting van mRNA Decay tarieven in

Article DOI: 10.3791/52240-v • 12:21 min • December 13th, 2014
December 13th, 2014 •
1, 1
1Department of Biology, Baylor University

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Het steady state niveau van specifieke mRNA's wordt bepaald door de snelheid van synthese en afbraak van het mRNA. Genoom-brede mRNA degradatie tarieven of de vervalsnelheid van specifieke mRNA's kan worden gemeten door het bepalen van mRNA halfwaardetijden. Dit protocol richt zich op het meten van mRNA vervalsnelheden in Saccharomyces cerevisiae.

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Cellular Biology , MRNA verval mRNA stabiliteit-nonsense gemedieerde mRNA verval mRNA halfwaardetijd transcriptie remming
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