Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Joshua W. Schaeffer; Jeffrey C. Chandler; Margaret Davidson; Sheryl L. Magzamen; Alma P&#233;rez-M&#233;ndez; Stephen J. Reynolds; Lawrence D. Goodridge; John Volckens; Alan B. Franklin; Susan A. Shriner; Bledar Bisha

doi:10.3791/58111

Journal

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Environment

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Detection of Viruses from Bioaerosols Using Anion Exchange Resin

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Detection of Viruses from Bioaerosols Using Anion Exchange Resin

8,237 Views

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06:10 min

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August 22, 2018

DOI:

10.3791/58111-v

DOI:

10.3791/58111-v

•

06:10 min

August 22, 2018

•

8218 Views

Joshua W. Schaeffer¹, Jeffrey C. Chandler², Margaret Davidson^1,3, Sheryl L. Magzamen¹, Alma Pérez-Méndez⁴, Stephen J. Reynolds¹, Lawrence D. Goodridge⁵, John Volckens⁶, Alan B. Franklin², Susan A. Shriner², Bledar Bisha⁷

¹High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences,Colorado State University ²National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service,United States Department of Agriculture ³Western Sydney University ⁴Leprino Foods, Inc ⁵Department of Food Science and Agricultural Chemistry,McGill University ⁶Department of Mechanical Engineering,Colorado State University ⁷Department of Animal Science,University of Wyoming

Summary

An anion exchange resin-based method, adapted to liquid impingement-based bioaerosol sampling of viruses is demonstrated. When coupled with downstream molecular detection, the method allows for facile and sensitive detection of viruses from bioaerosols.