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Detection of Viruses from Bioaerosols Using Anion Exchange Resin
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Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

DOI:
10.3791/58111-v

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06:10 min

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August 22, 2018

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, , , , , , , , , ,
1High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences,Colorado State University, 2National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service,United States Department of Agriculture, 3Western Sydney University, 4Leprino Foods, Inc, 5Department of Food Science and Agricultural Chemistry,McGill University, 6Department of Mechanical Engineering,Colorado State University, 7Department of Animal Science,University of Wyoming

Chapters

  • 00:04Title
  • 00:30Bioaerosol Chamber Setup and Model Bioaerosolization Experiments
  • 02:58Nucleic Acid Extraction and qRT-PCR Detection
  • 04:10Results: Anion Exchange Resin Improves qRT-PCR-based, Negatively-charged Virus Bioaerosol Detection
  • 04:46Conclusion

Summary

Automatic Translation

Automatic Translation

An anion exchange resin-based method, adapted to liquid impingement-based bioaerosol sampling of viruses is demonstrated. When coupled with downstream molecular detection, the method allows for facile and sensitive detection of viruses from bioaerosols.

Tags

Keywords: Virus DetectionBioaerosolsAnion Exchange ResinMolecular DetectionPublic HealthVeterinary HealthViral ParticlesCollision NebulizerLiquid ImpingersHEPA Filtered AirAir ContaminantsSamplingCalibration

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