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Chemistry

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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein
 

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Article doi: 10.3791/52404
April 2nd, 2015

Summary April 2nd, 2015

Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).

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