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Седиментационного равновесия небольшой олигомер-формирования мембранного белка: Влияние гистидин ПРОТОНИРОВАНИЕ на пентамерного стабильности
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
DOI:

09:49 min

April 02, 2015

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Chapters

  • 00:05Title
  • 01:48Density Matching of Detergent Miscelles with 2H2O
  • 05:37Sedimentation Equilibrium of SH Protein in C14 Sulfobetaine Micelles
  • 08:30Results: Effect of pH on SH Protein Pentameric Stability
  • 09:08Conclusion

Summary

Automatic Translation

Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).

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