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Immunology and Infection

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JoVE Journal Immunology and Infection
Исследование тучных клеток секреторных гранул; от биосинтеза к экзоцитозу
 
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Исследование тучных клеток секреторных гранул; от биосинтеза к экзоцитозу

Article doi: 10.3791/52505
January 26th, 2015 •
1,2, 3, 2, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

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January 26th, 2015

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Целью настоящего протокола заключается в разработке способа, который позволит функциональные геномные анализы секреции тучных клеток. Протокол основан на количественной оценке высвобождения флуоресцентного гена-репортера cotrasfected с интересующего гена и реального времени анализа морфологии секреторной гранулы в.

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