Isolatie van primaire humane Colon tumorcellen uit Chirurgische weefsels en kweken ze rechtstreeks op zachte elastische substraten voor Traction Cytometry
A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.