Isolierung von primären humanen Colon Tumorzellen von Surgical Gewebe und Kultivierung sie direkt auf weiche elastische Substrate für Traction Cytometry
A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.