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JoVE Journal Developmental Biology
Viral-mediert Merking og transplantasjon av Medial ganglieblokkere Eminence (MGE) Cells for
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Viral-mediert Merking og transplantasjon av Medial ganglieblokkere Eminence (MGE) Cells for

Article DOI: 10.3791/52740-v • 09:21 min • April 23rd, 2015
April 23rd, 2015 •
1, 1, 2, 2, 2, 1
1Department of Psychiatry, University of California San Francisco, 2Department of Neurological Surgery, University of California San Francisco

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Gabaergic kortikal Interneuron stamfedre spre seg, utvikle og postsynaptisk integrere i en verts cortex etter transplantasjon. Disse cellene kan lett omformet før transplantasjon for in vivo studier av genmodifiserte gabaergic forløpere. Her viser vi viral merking teknikker for å målrette bestemte Interneuron undergrupper ved hjelp av eksisterende Grobunn linjer og Cre-avhengige journalister.

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Developmental Biology MGE interneuron transplantasjon lentivirus celle merking somatostatin Cre
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