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Developmental Biology

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JoVE Journal Developmental Biology
Viral-medieret Mærkning og transplantation af Medial ganglioniske Eminence (MGE) Celler til
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
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Viral-medieret Mærkning og transplantation af Medial ganglioniske Eminence (MGE) Celler til

Article DOI: 10.3791/52740-v • 09:21 min • April 23rd, 2015
April 23rd, 2015 •
1, 1, 2, 2, 2, 1
1Department of Psychiatry, University of California San Francisco, 2Department of Neurological Surgery, University of California San Francisco

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GABAerge kortikal interneuron stamfædre sprede, udvikle og synaptisk integrere i et væld cortex efter transplantation. Disse celler kan let transduceres før transplantation til in vivo undersøgelser af genetisk modificerede GABAerge forstadier. Her viser vi virale mærkningsteknikker at målrette specifikke interneuron undergrupper under anvendelse af eksisterende Cre linjer og Cre-afhængige journalister.

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Developmental Biology MGE interneuron transplantation lentivirus celle mærkning somatostatin ska
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