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Immunology and Infection

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从大鼠睾丸支持细胞的分离和管周细胞

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Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

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从大鼠睾丸支持细胞的分离和管周细胞

DOI:

10.3791/53389-v

•

11:11 min

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February 08, 2016

•

Sudhanshu Bhushan, Ferial Aslani, Zhengguo Zhang, Tim Sebastian, Hans-Peter Elsässer, Jörg Klug

¹Institut für Anatomie und Zellbiologie,Justus-Liebig-Universität Gießen, ²Institut für Zytobiologie und Zytopathologie,Philipps-Universität Marburg

Chapters

00:05Title
01:26Seminiferous Tubule Preparation
04:41Peritubular Cell (PTC) Isolation
06:30Sertoli Cell Isolation
08:37Results: Representative PTC and Sertoli Cell Culture
09:31Conclusion

Summary

Automatic Translation

需要进行其免疫特性的炎症或感染，和利用在学习睾丸免疫特权，信号转导原塞尔托利细胞。在这里，我们描述了高度纯化的主支持细胞和从大鼠睾丸管周细胞的分离的基于酶的协议。

Tags

Sertoli Cells Peritubular Cells Rat Testes Cell Isolation Testicular Macrophages Autophagy Male Infertility Seminiferous Tubules Cell Culture Primary Cells

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