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水在油乳剂：与疏水性颜料装配水溶性叶绿素结合蛋白的新系统

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Chemistry

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JoVE Journal Chemistry

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

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水在油乳剂：与疏水性颜料装配水溶性叶绿素结合蛋白的新系统

DOI:

10.3791/53410-v

•

11:26 min

•

March 21, 2016

•

Dominika Bednarczyk, Dror Noy

¹Department of Biological Chemistry,Weizmann Institute of Science, ²Migal-Galilee Research Institute

Chapters

00:05Title
00:49Prepare Chlorophyll a Stock Solutions
04:11Prepare Organic and Aqueous Phases of Emulsion
06:43Assembly of WSCP with Chlorophyll a in Emulsion
09:05Results: Assembly of WSCP Apoproteins Using Water in Oil Emersions
10:15Conclusion

Summary

Automatic Translation

该原稿描述用于装配与基于水包油乳液的疏水颜料的水溶性蛋白质的简单和高通量方法。我们证明该方法在本地叶绿素大会的有效性与重组水溶性叶绿素的四种变体结合蛋白（WSCPs）的芸苔属植物在大肠杆菌中表达大肠杆菌 。

Tags

Keywords: Water In Oil Emulsions Chlorophyll-binding Proteins Hydrophobic Pigments Chlorophyll Extraction Spirulina Platensis Cyanobacteria DEAE-Sepharose Acetone Methanol Diethyl Ether Protein-pigment Complexes

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