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Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
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JoVE Journal Chemistry
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

DOI:
10.3791/53448-v

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08:01 min

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December 15, 2015

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, , , , ,
1School of Science and Health,University of Western Sydney

Chapters

  • 00:05Title
  • 01:15Setup of High Performance Liquid Chromatography (HPLC) Instrument and UV-Vis/Mass Spectrometry (MS) Detectors
  • 02:51Preparation of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Radical Reagent and System Detector
  • 04:00Setup of Parallel Segmented Flow (PSF) Column
  • 05:00Tuning the PSF Column for Multiplexed Detection
  • 06:18Results: Multiplexed Detection of a Coffee Sample
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the operation and tuning of parallel segmented flow chromatography columns to enable multiplexed detection.

Tags

Keywords: Active Flow TechnologyParallel Segmented Flow ColumnMultiplexed DetectionFlow HeterogeneitySeparation PerformanceEfficiencySensitivityColumn Outlet End-fittingDestructive And Non-destructive DetectorsPressure ManagementTube DimensionsFlow Adjustment

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