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Curtain Flow Column: Optimization of Efficiency and Sensitivity
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JoVE Journal Chemistry
Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

DOI:
10.3791/53471-v

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06:44 min

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June 12, 2016

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, , , , ,
1School of Science and Health,University of Western Sydney

Chapters

  • 00:05Title
  • 01:04Split-flow System Setup
  • 02:01Tuning of Curtain Flow (CF) Inlet Ratio on Split-flow System
  • 03:30Tuning of CF Outlet Flow
  • 04:56Results: CF Chromatography Increases Sensitivity Compared to Conventional Chromatography
  • 05:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the operation and optimization of Active Flow Technology (AFT) column in Curtain flow (CF) mode for enhanced separation performance.

Tags

Keywords: Curtain Flow ColumnSeparation EfficiencySeparation SensitivityHPLCMobile Phase OptimizationFlow Ratio OptimizationCentral PortPeripheral PortTrace Sample AnalysisEnvironmental SciencesForensics

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