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JoVE Journal Developmental Biology
Isolering och kultur av vuxna Zebrafish Brain-härledda Neuro
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Isolering och kultur av vuxna Zebrafish Brain-härledda Neuro

Article DOI: 10.3791/53617-v • 11:01 min • February 29th, 2016
February 29th, 2016 •
*1,2, *3, 1, 1,3, 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
* These authors contributed equally

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Här ger vi en reproducerbar metod för att undersöka vuxen neurogenes med hjälp av en neurosphere analys härrör från hela hjärnan eller från antingen telencephalic, tectal eller cerebellära regioner i vuxen zebrafisk hjärnan. Dessutom beskriver vi proceduren för att manipulera genuttrycket i zebrafisk neurosfärer.

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Utvecklingsbiologi Zebrafish neurogenes neurala adulta stamceller / progenitorceller neurosfären analysen miRNA genmanipulation
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