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JoVE Journal Neuroscience
Ex Vivo optogenetic Dissektion af Frygt Kredsløb i Brain Skiver
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Ex Vivo optogenetic Dissektion af Frygt Kredsløb i Brain Skiver

Article DOI: 10.3791/53628-v • 11:13 min • April 5th, 2016
April 5th, 2016 •
1, 2, 1
1Hertie Institute for Clinical Brain Research and Werner Reichardt Centre for Integrative Neuroscience, University of Tuebingen, 2Max Planck Florida Institute for Neuroscience

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Optogenetic tilgange er meget udbredt til at manipulere neurale aktivitet og vurdere konsekvenserne for hjernens funktion. Her er en teknik, skitseret, at ved in vivo-ekspression af det optiske aktivator Channelrhodopsin, giver mulighed for ex vivo analyse af synaptiske egenskaber af særlig lang rækkevidde og lokale neurale forbindelser i frygt-relaterede kredsløb.

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Neuroscience optogenetics Channelrhodopsin stereotaktisk injektion hel-celle patch-clamp amygdala mediale præfrontale cortex thalamus synapser neurale tilslutningsmuligheder
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