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Forbereder frisk Retinal skiver fra voksen zebrafisk for Ex Vivo billeddannelse eksperimenter
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
DOI:

11:01 min

May 09, 2018

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Chapters

  • 00:04Title
  • 00:44Preparing Animals and Equipment
  • 02:55Dissecting the Retina
  • 04:36Flat-mounting the Retina
  • 06:21Slicing the Retina and Assembling the Imaging Ladder
  • 08:39Results: Visualizing Ca2-dynamics in Cone Photoreceptors
  • 10:19Conclusion

Summary

Automatic Translation

Imaging retinale væv kan give én celle oplysninger, der ikke kan blive indsamlet fra traditionelle biokemiske metoder. Denne protokol beskriver forberedelse af retinale skiver fra zebrafisk for Konfokal billeddannelse. Fluorescerende kodet genetisk sensorer eller indikator farvestoffer tillade visualisering af mange biologiske processer i forskellige retinal celletyper.

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