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Genomförandet av en enhetlig Anti-Stokes Ramanspridning (CARS) System på en Ti: Sapphire och OPO laserbaserade Standard Laserskanning Mikroskop
JoVE Journal
Biology
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JoVE Journal Biology
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
DOI:

12:54 min

July 17, 2016

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Chapters

  • 00:05Title
  • 00:57Laser System Startup and Microscope Setup
  • 02:13Temporal Synchronization: Part 1
  • 04:25Temporal Synchronization: Part 2
  • 06:46Spatial Overlap of the Beams
  • 08:42Final Adjustments for CARS Signal Observation
  • 10:32Results: Myelin Sheath and Collagen Fibers Viewed by CARS
  • 12:05Conclusion

Summary

Automatic Translation

Sammanhängande anti-Stokes Ramanspridning (CARS) mikroskopi baserad på inneboende vibrationer molekyl obligationer tillåter etikett fri kemiskt selektiv levande cell imaging. Detta arbete presenteras genomförandet av en kompletterande mikroskopi teknik på en vanlig multifotonlaserskanning mikroskop baserad på en femtosekund Ti: safir laser och en OPO laser.

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