Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
 

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Article DOI: 10.3791/62398-v 12:08 min February 14th, 2022
February 14th, 2022

Chapters

Summary

This protocol presents rapid antimicrobial susceptibility testing (AST) assay within 2.5 h by single-cell-stimulated Raman scattering imaging of D2O metabolism. This method applies to bacteria in the urine or whole blood environment, which is transformative for rapid single-cell phenotypic AST in the clinic.

Transcript

Tags

Rapid Antimicrobial Susceptibility Testing Stimulated Raman Scattering Imaging Deuterium Incorporation Single Bacterium Analysis Time Reduction Broth Microdilution Method Bacterial Metabolic Activity Optical Density Measurement Bacterial Concentration MHB Medium Antibiotic Stock Solution Final Antibiotic Concentration Twofold Serial Dilution Test Antibiotics
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter