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蓝宝石OPO基于激光的标准激光扫描显微镜:在TI相干反斯托克斯拉曼散射(CARS)系统的实现
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JoVE Journal
Biology
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
Please note that all translations are automatically generated.
Click here for the English version.
蓝宝石OPO基于激光的标准激光扫描显微镜:在TI相干反斯托克斯拉曼散射(CARS)系统的实现
DOI:
10.3791/54262-v
•
12:54 min
•
July 17, 2016
•
Vasyl Mytskaniuk*
1
,
Fabrice Bardin*
2,3
,
Hassan Boukhaddaoui
5
,
Herve Rigneault
,
Nicolas Tricaud
1
INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier
,
2
Université de Nîmes
,
3
CNRS, IES, UMR 5214
,
4
Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249
,
5
Montpellier RIO Imaging (MRI)
Chapters
00:05
Title
00:57
Laser System Startup and Microscope Setup
02:13
Temporal Synchronization: Part 1
04:25
Temporal Synchronization: Part 2
06:46
Spatial Overlap of the Beams
08:42
Final Adjustments for CARS Signal Observation
10:32
Results: Myelin Sheath and Collagen Fibers Viewed by CARS
12:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
根据分子键固有振动相干反斯托克斯拉曼散射(CARS)显微允许无标记化学选择性活细胞成像。这项工作提出了一种基于飞秒钛互补显微技术在标准的多光子激光扫描显微镜实现:蓝宝石激光器和激光OPO。
Tags
Coherent Anti-Stokes Raman Scattering (CARS)
Ti:Sapphire Laser
OPO Laser
Laser Scanning Microscopy
Label-free Imaging
Chemically Selective Imaging
Lipid-related Diseases
Skin Penetration
Skin Disorders
Dichroic Mirror
Narrow Band Pass Filter
PMT
Fluorescence
SHG
Delay Line
Photo Diode
Oscilloscope
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