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Journal / Biochemistry / Cryomilled memeli hücrelerinden protein kompleksi …
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Biochemistry

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JoVE Journal Biochemistry
Cryomilled memeli hücrelerinden protein kompleksi olan ele geçirme kapasitesini
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Cryomilled memeli hücrelerinden protein kompleksi olan ele geçirme kapasitesini

Article DOI: 10.3791/54518-v • 10:37 min • December 9th, 2016
December 9th, 2016 •
1,2, 1, 1
1Laboratory of Cellular and Structural Biology, The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine

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Burada, bir kriyojenik sıcaklıkta katı hal öğütme ile memeli hücrelerin bozulması Elde edilen hücre tozu, bir hücre, ekstrenin üretilmesi ve antikor-bağlı mikron çaplı paramanyetik taneler üzerine afinite yakalama ile ilgili protein kompleksleri izole etmek için protokoller tarif eder.

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Biochemistry Sayı 118 afinite yakalama afinite saflaştırma immünopresipitasyon IP cryomilling hücre parçalanması protein kompleksi saflaştırma
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