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微电极阵列和膜片钳记录在人诱导多能干细胞来源心肌细胞上的技术应用
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JoVE Journal Medicine
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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微电极阵列和膜片钳记录在人诱导多能干细胞来源心肌细胞上的技术应用

DOI:
10.3791/64265-v

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10:30 min

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August 04, 2022

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, , , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Division of Cardiovascular Medicine, Department of Medicine,Stanford University School of Medicine, 3Department of Radiology,Stanford University School of Medicine

Chapters

  • 00:04Introduction
  • 00:56Measurement of hiPSC‐CM Contraction Motion
  • 01:59Measurement of hiPSC‐CM Field Potential
  • 04:16Measurement of hiPSC‐CM Action Potential
  • 06:22Measurement of hiPSC‐CM Ca2+ Transient
  • 08:09Results: Contraction Motion, Microelectrode Array, Patch Clamp Recordings and Ca2+ Imaging on hiPSC‐CMs
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

人诱导的多能干细胞来源的心肌细胞（hiPSC-CMs）已成为药物诱导的心脏毒性筛查和疾病建模的有前途的 体外 模型。在这里，我们详细介绍了用于测量hiPSC-CMs的收缩力和电生理学的方案。

Tags

Human IPSC-derived CardiomyocytesCardiac Safety AssessmentPre-clinical EvaluationMicroelectrode ArrayPatch ClampDisease ModelingDrug-induced Cardiotoxicity ScreeningPrecision MedicineCell CultureExtracellular MatrixCell SeedingSpontaneous Beating

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