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JoVE Journal
Immunology and Infection

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SILAC Based proteomiske Karakterisering af Exosomer fra HIV-1 inficerede celler
 
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SILAC Based proteomiske Karakterisering af Exosomer fra HIV-1 inficerede celler

Article doi: 10.3791/54799
March 3rd, 2017

Summary March 3rd, 2017

Please note that all translations are automatically generated.

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Her beskriver vi en kvantitativ proteomics metode ved hjælp af teknikken af ​​stabile isotoper mærkning af aminosyrer i cellekultur (SILAC) at analysere virkningerne af HIV-1 infektion på host exosomal proteomer. Denne protokol kan let tilpasses til celler under forskellige stress eller infektion betingelser.

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