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Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
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Chemistry
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JoVE Journal Chemistry
Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

DOI:
10.3791/54885-v

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09:16 min

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January 09, 2017

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,
1Department of Chemistry, School of Science,University of Tokyo, 2Institute for Solid State Physics,University of Tokyo

Chapters

  • 00:05Title
  • 00:36Overview of the Dynamic Light Scattering Microscope
  • 01:30Sample Preparation: Temperature-responsive Polymer Solution
  • 03:32Preparation of the Sample Mounts
  • 04:47Optimization of the Instrument
  • 06:19Sample Measurement
  • 07:13Results: Size Distributions for Poly(Nisopropylacrylamide) (PNIPA) and a Polystyrene Latex Suspension
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the direct measurement of particle size distribution in concentrated solutions using dynamic light scattering microscopy is presented.

Tags

Keywords: Particle Size DistributionTurbid SolutionsDynamic Light ScatteringConfocal MicroscopyLaserAvalanche PhotodiodeAutocorrelatorInverted MicroscopeTemperature RegulatorBack Scattering GeometryCCD CameraNIPATMEDAAmmonium PersulfateArgon Gas FlowStirrerIce Bath

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