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Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
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JoVE Journal Medicine
Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

DOI:
10.3791/55178-v

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08:58 min

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March 11, 2017

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, , , ,
1CAS Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences,Chinese Academy of Sciences, 2Suzhou Niumag Analytical Instrument Corporation

Chapters

  • 00:05Title
  • 01:20Tissue Harvesting
  • 01:52Oil Red O Staining and Mounting
  • 04:16Primary Mouse Hepatocyte Model
  • 05:23Purifying Lipids from Liver Tissue
  • 07:18Results: Analysis of Whole Liver Tissue after HFHS Dieting and Analysis of Treated Hepatocyte Cultures
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

Here, optimized methods to generate in vivo and in vitro models of hepatic steatosis and to analyze the steatotic phenotypes and related physiological parameters are described.

Tags

Keywords: Hepatic SteatosisIn VivoIn VitroLipid MetabolismGlucose MetabolismFatty Liver DiseaseInsulin ResistanceType 2 DiabetesObesityHistological AnalysisBiochemical AnalysisOil Red O StainingCryosectioningLipid Content Measurement

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