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Journal
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Bioengineering
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一个<em>体外</em鼠类的椎间盘>器官培养模型
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
An
In Vitro
Organ Culture Model of the Murine Intervertebral Disc
Please note that all translations are automatically generated.
Click here for the English version.
一个<em>体外</em鼠类的椎间盘>器官培养模型
DOI:
10.3791/55437-v
•
08:03 min
•
April 11, 2017
•
Jennifer W. Liu
3
,
Kevin H. Lin
,
Christian Weber
,
Sameer Bhalla
,
Sean Kelso
,
Kaixi Wang
,
Simon Y. Tang
3,4
1
Department of Biomedical Engineering
,
Washington University in St. Louis
,
2
Department of Biology
,
Washington University in St. Louis
,
3
Department of Orthopaedic Surgery
,
Washington University in St. Louis
,
4
Department of Materials Science and Mechanical Engineering
,
Washington University in St. Louis
Chapters
00:05
Title
00:57
Animals and Dissection Protocol
02:47
Organ Culture Conditions
04:11
Longitudinal Measurements of NF-κB
04:39
Mechanical Assessment
05:57
Results: Properties of
In Vitro
-cultured IVDs
07:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
椎间盘(IVD)的整个器官培养保留天然的细胞外基质,细胞的表型,和细胞 - 基质相互作用。在这里我们将介绍使用鼠标腰部,并在其脊柱功能单位尾椎的IVD并利用该系统的几个应用的IVD文化体系。
Tags
In Vitro Organ Culture
Intervertebral Disc
Murine
Low Back Pain
Disc Degeneration
BALB/c Mice
Transgenic Mice
Luciferase Gene
Functional Spine Units
DMEM/F-12 Medium
Organ Culture Method
Dissection
Sterility
Contamination
Article
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