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en<em> In vitro</em> Orgel Kultur Model af muse diskusprolaps
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Bioengineering
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JoVE Journal Bioengineering
An In Vitro Organ Culture Model of the Murine Intervertebral Disc

en<em> In vitro</em> Orgel Kultur Model af muse diskusprolaps

DOI:
10.3791/55437-v

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08:03 min

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April 11, 2017

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, , , , , ,
1Department of Biomedical Engineering,Washington University in St. Louis, 2Department of Biology,Washington University in St. Louis, 3Department of Orthopaedic Surgery,Washington University in St. Louis, 4Department of Materials Science and Mechanical Engineering,Washington University in St. Louis

Chapters

  • 00:05Title
  • 00:57Animals and Dissection Protocol
  • 02:47Organ Culture Conditions
  • 04:11Longitudinal Measurements of NF-κB
  • 04:39Mechanical Assessment
  • 05:57Results: Properties of In Vitro-cultured IVDs
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

Hel organkultur af diskusprolaps (IVD) bevarer det native ekstracellulære matrix, cellefænotyper, og cellulær-matrix-interaktioner. Her beskriver vi et IVD kultur-system ved hjælp af musen lumbal og caudale IVDs i deres funktionelle spinal enheder og flere programmer udnytter dette system.

Tags

In Vitro Organ CultureIntervertebral DiscMurineLow Back PainDisc DegenerationBALB/c MiceTransgenic MiceLuciferase GeneFunctional Spine UnitsDMEM/F-12 MediumOrgan Culture MethodDissectionSterilityContamination

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