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An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Bioengineering
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JoVE Journal Bioengineering
An In Vitro Organ Culture Model of the Murine Intervertebral Disc

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

DOI:
10.3791/55437-v

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08:03 min

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April 11, 2017

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, , , , , ,
1Department of Biomedical Engineering,Washington University in St. Louis, 2Department of Biology,Washington University in St. Louis, 3Department of Orthopaedic Surgery,Washington University in St. Louis, 4Department of Materials Science and Mechanical Engineering,Washington University in St. Louis

Chapters

  • 00:05Title
  • 00:57Animals and Dissection Protocol
  • 02:47Organ Culture Conditions
  • 04:11Longitudinal Measurements of NF-κB
  • 04:39Mechanical Assessment
  • 05:57Results: Properties of In Vitro-cultured IVDs
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.

Tags

In Vitro Organ CultureIntervertebral DiscMurineLow Back PainDisc DegenerationBALB/c MiceTransgenic MiceLuciferase GeneFunctional Spine UnitsDMEM/F-12 MediumOrgan Culture MethodDissectionSterilityContamination

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