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An In Vitro Organ Culture Model of the Murine Intervertebral Disc
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
DOI:

08:03 min

April 11, 2017

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Chapters

  • 00:05Title
  • 00:57Animals and Dissection Protocol
  • 02:47Organ Culture Conditions
  • 04:11Longitudinal Measurements of NF-κB
  • 04:39Mechanical Assessment
  • 05:57Results: Properties of In Vitro-cultured IVDs
  • 07:07Conclusion

Summary

Automatic Translation

Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.

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