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Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Bioengineering
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JoVE Journal Bioengineering
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

DOI:
10.3791/55438-v

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04:40 min

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July 02, 2017

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, , , , , , , , , , ,
1Division of Cardiac Surgery,Johns Hopkins Hospital, 2Division of Cardiology,Johns Hopkins Hospital, 3Department of Biomedical Engineering,Johns Hopkins University

Chapters

  • 00:05Title
  • 00:593D Bioprinting of Spheroids
  • 02:16Removal of Patch from the Needle Array and Patch Maturation
  • 03:35Results: 3D Bioprinted Spheroids Exhibit Mechanical Integration
  • 03:55Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.

Tags

3D BioprintingSpheroidsCardiomyocytesEndothelial CellsFibroblastsCell IsolationCell MixingUltra-low Attachment PlatesSpheroid FormationConfocal MicroscopyConnexin 43Troponin T3D BioprinterNeedle ArrayVacuum SuctionCardiac PatchSterile ConditionsPhosphate Buffered SalineCell Culture Media

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