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Journal / Developmental Biology / Protokolle für die Visualisierung Steroidogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Protokolle für die Visualisierung Steroidogenic Organe und deren Interactive Organe mit Immunostaining in der Fruchtfliege
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Protokolle für die Visualisierung Steroidogenic Organe und deren Interactive Organe mit Immunostaining in der Fruchtfliege

Article DOI: 10.3791/55519-v • 12:10 min • April 14th, 2017
April 14th, 2017 •
*1, *1, *2, 3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, 3Faculty of Life and Environmental Sciences, University of Tsukuba
* These authors contributed equally

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Wir beschreiben ein Protokoll für die Präparation, Fixierung und Immunfärbung von steroidogenen Organen in Drosophila - Larven und erwachsenen Frauen Steroidhormon - Biosynthese und ihren Regulationsmechanismus zu studieren. Neben steroidogenen Organe visualisieren wir die Innervation steroidogenen Organe sowie steroidogenen Zielzellen wie Keimbahn-Stammzellen.

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Entwicklungsbiologie Heft 122 Entwicklungsbiologie Neurowissenschaften, Ecdysteroid-Biosynthese Immunhistochemie Keimbahn-Stammzellen Eierstock- Prothoraxdrüse
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