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协议的可视化类固醇机关及其互动器官在果蝇免疫染色<em>果蝇</em
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
DOI:

12:10 min

April 14, 2017

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Chapters

  • 00:05Title
  • 00:57Dissecting the Larval Ring Gland-brain Complex
  • 01:53Filleting Larvae to Maintain Relative Tissue Positions
  • 03:35Fine Dissection of the Brain-ring Gland Complex and Mounting
  • 05:34Dissection of Adult Ovary
  • 06:36Dissection of Adult Reproductive Organs Along with Other Interactive Tissues
  • 08:15Mounting of Adult Structures and Imaging
  • 09:33Results: Preparation images
  • 11:13Conclusion

Summary

Automatic Translation

我们描述了解剖,固定,并在果蝇幼虫和成年女性类固醇合成器官,研究类固醇激素的生物合成及其调控机制的免疫染色的协议。除了类固醇合成器官,我们可以形象地类固醇合成器官的神经支配和类固醇合成的靶细胞,如生殖系干细胞。

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