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Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
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JoVE Journal Developmental Biology
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

DOI:
10.3791/55519-v

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12:10 min

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April 14, 2017

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, , ,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance,University of Tsukuba, 3Faculty of Life and Environmental Sciences,University of Tsukuba

Chapters

  • 00:05Title
  • 00:57Dissecting the Larval Ring Gland-brain Complex
  • 01:53Filleting Larvae to Maintain Relative Tissue Positions
  • 03:35Fine Dissection of the Brain-ring Gland Complex and Mounting
  • 05:34Dissection of Adult Ovary
  • 06:36Dissection of Adult Reproductive Organs Along with Other Interactive Tissues
  • 08:15Mounting of Adult Structures and Imaging
  • 09:33Results: Preparation images
  • 11:13Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for dissection, fixation, and immunostaining of steroidogenic organs in Drosophila larvae and adult females to study steroid hormone biosynthesis and its regulatory mechanism. In addition to steroidogenic organs, we visualize the innervation of steroidogenic organs as well as steroidogenic target cells such as germline stem cells.

Tags

Keywords: Drosophila MelanogasterSteroidogenic OrgansImmunostainingDissectionLarvaBrain-ring Gland ComplexFat BodySalivary GlandsFixative SolutionPBS

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