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Novel analyse for Cold Nociception i<em> Drosophila</em> Larver
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JoVE Journal Neuroscience
Novel Assay for Cold Nociception in Drosophila Larvae

Novel analyse for Cold Nociception i<em> Drosophila</em> Larver

DOI:
10.3791/55568-v

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06:52 min

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April 03, 2017

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, ,
1Department of Genetics,UT MD Anderson Cancer Center, 2Neuroscience Program,Graduate School of Biomedical Sciences at Houston, 3ProDev Engineering, 4Genes and Development Program,Graduate School of Biomedical Sciences at Houston, 5Section of Neurobiology,University of Southern California

Chapters

  • 00:05Title
  • 00:56Preparation of Larvae
  • 02:31Cold Probe Assay
  • 04:36Results: Larvae Exhibit Unique Behaviors in Response to a Noxious Cold Stimulus
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

Her demonstrerer vi en hidtil ukendt assay til at studere kold nociception i Drosophila larver. Dette assay anvender en specialbygget Peltier probe stand til at påføre en fokal skadelig kold stimulus og resulterer i kvantificerbare kolde-specifikke adfærd. Denne teknik vil muliggøre yderligere cellulær og molekylær dissektion af kold nociception.

Tags

Drosophila LarvaeCold NociceptionNoxious Cold DetectionCold StimulusCold-evoked ResponsesGenetic ContextsThird Instar LarvaeCold ProbeTemperatureNociceptionLarvae SortingSample Preparation

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