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JoVE Journal
Genetics

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In Vitro Bioluminescentie Assay te karakteriseren Circadian Rhythm in borstklier epitheliale cellen
 
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In Vitro Bioluminescentie Assay te karakteriseren Circadian Rhythm in borstklier epitheliale cellen

Article DOI: 10.3791/55832
September 28th, 2017

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Summary September 28th, 2017

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Een in vitro bioluminescentie test om te bepalen van cellulaire circadiane ritme in de borstklier epitheliale cellen wordt gepresenteerd. Deze methode maakt gebruik van zoogdiercellen verslaggever plasmiden gedestabiliseerd luciferase onder de controle van de periode 2 gen promotor uitdrukken. Het kan worden aangepast aan andere celtypes te evalueren orgel-specifieke effecten op de circadiane ritme.

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