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En metode til vurdering af bakterieocin-virkninger på musens tarmmikrobiotikum
JoVE Journal
Biology
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JoVE Journal Biology
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
DOI:

07:54 min

July 25, 2017

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Chapters

  • 00:05Title
  • 00:40Preparation of Bacterial Cultures to Inoculate Mice
  • 01:46Mice Assay and Experimental Design and Collecting Samples
  • 03:08LAB Counting and Bacteriocin Activity
  • 04:39DNA Extraction, 16S rDNA Amplification, and Sequencing
  • 05:45Results: Detection and Comparison of Bacteriocin Production in Lactic Acid Bacteria Recovered from Feces
  • 07:12Conclusion

Summary

Automatic Translation

Bakteriociner menes at spille en central rolle i at definere mikrobiell mangfoldighed i forskellige økologiske nicher. Her beskriver vi en effektiv procedure til vurdering af, hvordan bakteriociner påvirker tarmmikrobiotasammensætningen i en dyremodel.

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