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Biology
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评估细菌素对小鼠肠道微生物的影响的方法
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Biology
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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Click here for the English version.
评估细菌素对小鼠肠道微生物的影响的方法
DOI:
10.3791/56053-v
•
07:54 min
•
July 25, 2017
•
Chrstine Bäuerl*
1
,
Özgun C.O. Umu*
2
,
Pablo E. Hernandez
,
Dzung B. Diep
,
Gaspar Pérez-Martínez
1
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA)
,
Consejo Superior de Investigaciones Científicas (CSIC)
,
2
Department of Food Safety and Infection Biology
,
Norwegian University of Life Sciences (NMBU)
,
3
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria
,
Universidad Complutense de Madrid (UCM)
,
4
Faculty of Chemistry, Biotechnology and Food Science
,
Norwegian University of Life Sciences (NMBU)
Chapters
00:05
Title
00:40
Preparation of Bacterial Cultures to Inoculate Mice
01:46
Mice Assay and Experimental Design and Collecting Samples
03:08
LAB Counting and Bacteriocin Activity
04:39
DNA Extraction, 16S rDNA Amplification, and Sequencing
05:45
Results: Detection and Comparison of Bacteriocin Production in Lactic Acid Bacteria Recovered from Feces
07:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
细菌素被认为在定义不同生态位的微生物多样性方面发挥关键作用。在这里,我们描述了一种有效的方法来评估细菌素如何影响动物模型中的肠道微生物群组成。
Tags
Gut Microbiota
Bacteriocins
Mouse Model
Probiotic
Bacterial Populations
Physiological Parameters
Host Organism
Serial Dilutions
Colony Forming Units
Drinking Water
Bacterial Survival
BALB/c Mice
Fecal Samples
Bacterial Suspension
Bacteriocin-producing Bacteria
MRS Soft Agar
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