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Biochemistry
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人的 ikkknα的生产、结晶和结构测定指南
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Please note that all translations are automatically generated.
Click here for the English version.
人的 ikkknα的生产、结晶和结构测定指南
DOI:
10.3791/56091-v
•
11:27 min
•
November 02, 2018
•
Smarajit Polley
2
,
De-Bin Huang
,
Tapan Biswas
,
Gourisankar Ghosh
1
Department of Chemistry & Biochemistry
,
University of California, San Diego
,
2
Department of Biophysics
,
Bose Institute
Chapters
00:05
Title
01:40
Preparation of Recombinant Virus Suitable for Large Scale Expression of IKK1/α
03:41
Purification of His-IKK1
05:54
Screen for Crystallization Condition of IKK1
06:47
Growing Crystals in Large Numbers
07:45
Cryo Protection of Crystals
08:57
Results: Crystallization, and Structure Determination of Human IKK1/α
10:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
b 激酶 1/α (ikkniα-chuk) 是一种 ser/thr 蛋白激酶, 主要通过激活 nf-b 转录因子参与无数的细胞活动。在这里, 我们描述了生产和晶体结构测定这种蛋白质所需的主要步骤。
Tags
IKK1-alpha
Human
Protein Expression
Insect Cells
Sf9 Cells
Transfection
Crystallization
Structure Determination
Molecular Replacement
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