Journal
/
/
En Guide til produktion, krystallisering og struktur bestemmelse af menneskelige IKK1/α
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
DOI:

11:27 min

November 02, 2018

, , ,

Chapters

  • 00:05Title
  • 01:40Preparation of Recombinant Virus Suitable for Large Scale Expression of IKK1/α
  • 03:41Purification of His-IKK1
  • 05:54Screen for Crystallization Condition of IKK1
  • 06:47Growing Crystals in Large Numbers
  • 07:45Cryo Protection of Crystals
  • 08:57Results: Crystallization, and Structure Determination of Human IKK1/α
  • 10:34Conclusion

Summary

Automatic Translation

IκB Kinase 1/α (IKK1/α CHUK) er en Ser/Thr protein kinase, der er involveret i et utal af cellulære aktiviteter primært gennem aktivering af NF-κB transkriptionsfaktorer. Her beskriver vi de vigtigste trin er nødvendige for produktionen og Krystalstrukturbestemmelse af dette protein.

Related Videos

Read Article