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Journal
/
Biology
/
实时
在体
记录了
拟南芥
钙信号在使用荧光传感器的昆虫饲喂时
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Real-time
In Vivo
Recording of
Arabidopsis
Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
Please note that all translations are automatically generated.
Click here for the English version.
实时
在体
记录了
拟南芥
钙信号在使用荧光传感器的昆虫饲喂时
DOI:
10.3791/56142-v
•
08:21 min
•
August 15, 2017
•
Thomas R. Vincent
,
James Canham
,
Masatsugu Toyota
3,4
,
Marieta Avramova
,
Sam T. Mugford
,
Simon Gilroy
,
Anthony J. Miller
,
Saskia Hogenhout
,
Dale Sanders
1
Department of Metabolic Biology
,
John Innes Centre, Norwich Research Park
,
2
Department of Botany
,
University of Wisconsin, Madison
,
3
Department of Biochemistry and Molecular Biology
,
Saitama University
,
4
Precursory Research for Embryonic Science and Technology (PRESTO)
,
Japan Science and Technology Agency (JST)
,
5
Department of Cell and Developmental Biology
,
John Innes Centre, Norwich Research Park
Chapters
00:05
Title
00:44
Arabidopsis
Leaf Detachment
01:38
Fluorescence Microscopy
03:06
Data Collection
05:39
Time-course Video Creation
06:39
Results: Analysis of GCaMP3 Fluorescence to Detect Aphid-elicited Calcium Signals at the Feeding Site in Detached
Arabidopsis
Leaves
07:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本议定书概述了喂养 hemipteran 的昆虫,如蚜虫等所产生的植物钙信号分析的简单方法。
拟南芥
转化与绿色荧光蛋白钙生物传感器 GCaMP3 允许实时
在体
成像的高时空分辨率的钙动力学。
Tags
Arabidopsis
Calcium Signals
Insect Feeding
Fluorescent Biosensor
In Vivo Recording
Plant-aphid Interactions
Calcium Signaling
GCaMP3
96-well Plate
Fluorescent Stereo Microscope
Image Analysis
Fiji
ImageJ
Article
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