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激光扫描显微术实时跟踪眼球前室胸腺细胞
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JoVE Journal Biology
Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy
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激光扫描显微术实时跟踪眼球前室胸腺细胞

DOI:
10.3791/58236-v

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08:21 min

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October 02, 2018

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,
1School of Medicine and Dentistry,Universidad Católica de Valencia San Vicente Mártir, 2Unidad Mixta CIPF-UCV,Centro de Investigación Príncipe Felipe, 3Department of Medicine,University of Miami

Chapters

  • 00:04Title
  • 00:51Newborn Thymus Isolation
  • 02:13Thymus Anterior Eye Chamber Implantation
  • 04:073D Single Photon Fluorescence Implanted Thymus Confocal Imaging
  • 06:17Results: Representative In Vivo Thymus Implant Imaging
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目的是通过激光扫描显微镜在小鼠眼前腔的胸腺植入物中显示胸腺细胞的纵向活体实时跟踪。角膜的透明度和移植的血管化允许持续记录祖细胞的招募和成熟的 T 细胞出口。

Tags

In Vivo TrackingThymocytesAnterior ChamberLaser Scanning MicroscopyAutoimmunityImmunodeficiencyCentral ToleranceThymus InvolutionProgenitor RecruitmentT-cell EgressionThymus TransplantationCorneal IncisionAnterior Chamber Implantation

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